PhD thesis
2024
Martin Wiinberg, MSc in Molecular Biomedicine, PhD, Dept. of Dermatology, Copenhagen University Hospital, Bispebjerg and Frederiksberg

Exploring Keratinocyte Carcinoma Models for Preclinical Treatment Evaluation

Aim

To develop an immunocompetent transplantable mouse models of keratinocyte carcinoma (BCC and cSCC) for preclinical drug development, and to evaluate their utility in testing novel immunotherapies and understanding treatment mechanisms.

Study objectives

  1. Model Development: Establish a transplantable syngeneic basal cell carcinoma (BCC) model derived from a genetically engineered mouse model & develop a transplantable syngeneic cutaneous squamous cell carcinoma (cSCC) model from an ultraviolet-induced cSCC model.
  2. Model Characterization: Compare the transplantable BCC model with its parental model to confirm resemblance in histological, immunological, and transcriptional features.
  3. Therapeutic Evaluation of TLR7/8 Agonist in Model: Utilize the cSCC model to evaluate the efficacy of intratumoral resiquimod sustained release gel - both alone and in combination with ablative fractional laser (AFL).
  4. Immunological Investigation of AFL: Investigate the immunological impact of AFL on macrophage polarization present in the skin. 

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